cytotoxicity assay造句

• 2 . cytotoxicity assay hela, skov-3, and wish cells were plated out in 96-well plates at a density of 1.0x104 cells per well in the serum-free rpmi-1640 medium
  值后发现和rl’a蛋白相比较，rta一yqrl细胞毒性作用明显提高，分别是前者的2，10和40倍。
• The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells; b, discrimination of target cells from effector cells in cell-mediated cytotoxicity assay; c, less treatment step, and free-radioactivity; d, high sensitivity and reliability . 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected . the results showed that the number of pbmc reduced, but the activity of pbmc increased dose-dependently; the reduction of cell number resulted from necrosis and apoptosis; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it; the ratio of t cell subset and the expression of thl and th2 cytokines increased
  结合以上创建的方法和免疫荧光流式细胞术，用k562细胞株可溶性分泌物(上清)对外周血单个核细胞(pbmc)进行培养以模拟体内微环境，然后分别从细胞增殖、凋亡、坏死、细胞周期、活性、细胞因子和表面抗原表达等方面进行研究，结果发现用肿瘤上清培养的pbmc细胞数量下降明显，但同时对其有激活作用，且呈剂量依赖性；细胞数的下降主要是由细胞坏死和凋亡引起的，肿瘤上清对细胞周期没有阻断作用，反而略有促进作用；t细胞亚群比例增加，并促进表达th1、th2细胞因子。